球体
间充质干细胞
细胞生物学
组织工程
移植
再生医学
干细胞
细胞培养
细胞
化学
生物医学工程
生物
医学
生物化学
外科
遗传学
作者
Elena Redondo‐Castro,Catriona Cunningham,Jonjo Miller,Stuart A. Cain,Stuart M. Allan,Emmanuel Pinteaux
出处
期刊:Bio-protocol
[Bio-Protocol]
日期:2018-01-01
卷期号:8 (16)
被引量:19
标识
DOI:10.21769/bioprotoc.2968
摘要
The 3D culture of human mesenchymal stem cells (hMSCs) represents a more physiological environment than classical 2D culture and has been used to enhance the MSC secretome or extend cell survival after transplantation. Here we describe a simple and affordable method to generate 3D spheroids of hMSCs by seeding them at high density in a low-binding 96-well plate. Spheroids of hMSCs cultured in low-binding 96-well plates can be used to study the basic biology of the cells and to generate conditioned media or spheroids to be used in transplantation therapeutic approaches. These MSCs or their secretome can be used as a regenerative therapy and for tissue repair across multiple disease areas, including neurodegeneration. In comparison to other methods (hanging drop, use of gels or biomaterials, magnetic levitation, etc.), the method described here is simple and affordable with no need to use specialized equipment, expensive materials or complex reagents.
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