小胶质细胞
IRF8
神经营养因子
脑源性神经营养因子
尼古丁
下调和上调
受体
内分泌学
内科学
生物
化学
免疫学
炎症
医学
基因表达
生物化学
基因
作者
Lijia Guo,Yang Zhang,Qing Lv,Zongwang Zhang
出处
期刊:Neuroreport
[Ovid Technologies (Wolters Kluwer)]
日期:2020-11-05
卷期号:31 (18): 1249-1255
被引量:4
标识
DOI:10.1097/wnr.0000000000001546
摘要
Objective Upregulation of P2X4 receptor (P2X4R), brain-derived neurotrophic factor (BDNF), and interleukin-1 beta (IL-1β) in activated microglia is associated with hyperalgesia. This study investigated whether nicotine increases pain hypersensitivity by altering the expression of these molecules in microglia. We also examined the role of interferon regulatory factor 8 (IRF8) in this process. Methods Experiments were performed in BV2 microglial cells. IRF8 was knocked down or overexpressed using lentiviruses harboring a short hairpin RNA targeting IRF8 or an IRF8 overexpression construct, respectively. P2X4R, BDNF, and IL-1β mRNA and protein levels were evaluated by real-time PCR and western blotting, respectively, and BDNF and IL-1β secretion was assessed by ELISA. Results Chronic nicotine exposure enhanced the expression of P2X4R, BDNF, and IL-1β in BV2 cells, and stimulated the release of BDNF and IL-1β in the presence of ATP. IRF8 was found to mediate the nicotine-induced increases in BDNF and IL-1β mRNA and P2X4R protein levels in BV2 cells. Conclusion Nicotine may increase pain hypersensitivity by promoting the expression of P2X4R, BDNF, and IL-1β through modulation of IRF8 levels in microglial cells.
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