丁酸钠
丁酸
丁酸盐
分泌物
紧密连接
化学
细胞生物学
炎症
内分泌学
胆汁酸
酒精性肝病
脂肪肝
封堵器
内科学
生物
生物化学
疾病
医学
基因
肝硬化
发酵
作者
Tingting Yang,Hao Yang,Heng Cai,Haiyan Wang,Shangxiu Chen,Yinlu Hu,Zhenzhou Jiang,Qiongna Yu,Zhongjian Wang,Sitong Qian,Jianyun Wang,Tao Wang,Lei Du,Qian Lü,Xiaoxing Yin
出处
期刊:Food & Function
[Royal Society of Chemistry]
日期:2020-01-01
卷期号:11 (12): 10675-10689
被引量:59
摘要
The intestinal microenvironment, a potential factor that contributes to the development of non-alcoholic fatty liver disease (NALFD) and type 2 diabetes (T2DM), has a close relationship with intestinal tight junctions (TJs). Here, we show that the disruption of intestinal TJs in the intestines of 16-week-old db/db mice and in high glucose (HG)-cultured Caco-2 cells can both be improved by sodium butyrate (NaB) in a dose-dependent manner in vitro and in vivo. Accompanying the improved intestinal TJs, NaB not only relieved intestine inflammation of db/db mice and HG and LPS co-cultured Caco-2 cells but also restored intestinal Takeda G-protein-coupled (TGR5) expression, resulting in up-regulated serum GLP-1 levels. Subsequently, the GLP-1 analogue Exendin-4 was used to examine the improvement of lipid accumulation in HG and free fatty acid (FFA) co-cultured HepG2 cells. Finally, we used 16-week-old db/db mice to examine the hepatoprotective effects of NaB and its producing strain Clostridium butyricum. Our data showed that NaB and Clostridium butyricum treatment significantly reduced the levels of blood glucose and serum transaminase and markedly reduced T2DM-induced histological alterations of the liver, together with improved liver inflammation and lipid accumulation. These findings suggest that NaB and Clostridium butyricum are a potential adjuvant treatment strategy for T2DM-induced NAFLD; their hepatoprotective effect was linked to the modulation of intestinal TJs, causing the restoration of glucose and lipid metabolism and the improvement of inflammation in hepatocytes.
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