Production of γ-Aminobutyric Acid and Antioxidative Effects by Levilactobacillus brevis

In this study, in order to isolate lactic acid bacteria with remarkable GABA-producing ability, twelve home-made Kimchi samples were collected in Korea. Among these Kimchi samples, only 4 Kimchi samples were showed the GABA spots on TLC plate. No. 9 (Chonggak-Kimchi), No. 11 (Yeolmu-Kimchi), No. 12 (Oi-So-Bagi), and No. 17 (Chonggak-Kimchi) Kimchi sample had GABA producer. From these selected Kimchi samples, 17 strains, lactic acid bacteria with GABA-producing ability, were isolated and identified. The results of 16s rRNA analysis showed more than 99% homologies with Levilactobacillus brevis ATCC 14869, respectively. According to the result of anti-oxidant activity, the DPPH radical scavenging ability was detected in the culture supernatant containing GABA by isolates, L. brevis BMSE-strains. Strain BMSE-G005 had the highest activity of 92.81%, Strain BMSE-G008, G010, G013, G016, G007, G004, G011, G012, G001 also showed more than 90% scavenging ability, respectively. Strain BMSE- G003, G017, G014, G002, G009, and G015 were confirmed to have an activity of 80% or more, respectively. Therefore, 17 strains isolated in this study were confirmed their potential as useful microbial resources with GABA-producing abilities and antioxidant effects.