化学
生物传感器
石墨烯
牛血清白蛋白
基质(水族馆)
纳米技术
酶
过氧化物酶
组合化学
色谱法
生物化学
海洋学
地质学
材料科学
作者
Niru Ye,Siming Huang,Huangsheng Yang,Tong Wu,Linjing Tong,Fang Zhu,Guosheng Chen,Gangfeng Ouyang
出处
期刊:Analytical Chemistry
[American Chemical Society]
日期:2021-10-04
卷期号:93 (41): 13981-13989
被引量:31
标识
DOI:10.1021/acs.analchem.1c03381
摘要
Nanozymes are of particular interest due to their enzyme-mimicking activity and high stability that are favorable in biomedical sensing and immunoassays. In this work, we report a highly specific N-doped nanozyme through pyrolysis of framework-confined bovine serum albumin (BSA). This strategy allows one to translate the low-cost and featureless BSA into a highly active enzyme mimic. The obtained carbon nanozyme (denoted as HBF-1-C800) displays 3- to 7-fold enhancement on peroxidase (POD) activity compared with the conventional carbon nanozymes and also shows ca. 5-fold activity enhancement compared to the reported N-doping graphene. Such excellent POD activity originates from high N-doping efficiency, protein-induced defective sites, and the intrinsic porous structure of HBF-1-C800, which provides abundantly accessible active sites and accelerates substrate diffusion simultaneously. Importantly, the HBF-1-C800 nanozyme has highly specific POD activity and also enables resistance to several harsh conditions that should denature natural enzymes. These features allow it with high accuracy, stability, and sensitivity for biosensing applications. Moreover, HBF-1-C800 has been designed as a promising platform for colorimetric biosensing of several biomarkers including H2O2, glutathione, and glucose, with wide linear ranges and low limits of detection that are satisfied with the disease diagnosis.
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