吲哚青绿
胰腺癌
肿瘤微环境
基质
材料科学
右旋糖酐
癌症研究
动态成像
胰腺肿瘤
医学
化学
癌症
病理
荧光寿命成像显微镜
荧光
内科学
生物化学
计算机科学
人工智能
图像处理
免疫组织化学
物理
图像(数学)
数字图像处理
量子力学
作者
Xinping Luo,Dehong Hu,Duyang Gao,Yuenan Wang,Xinhua Chen,Xin Liu,Hairong Zheng,Minjie Sun,Zonghai Sheng
出处
期刊:ACS Nano
[American Chemical Society]
日期:2021-06-01
卷期号:15 (6): 10010-10024
被引量:43
标识
DOI:10.1021/acsnano.1c01608
摘要
Tumor-associated macrophages (TAMs) play a crucial part in cancer evolution. Dynamic imaging of TAMs is of great significance for treatment outcome evaluation and precision tumor therapy. Currently, most fluorescence nanoprobes tend to accumulate in the liver and are difficult to metabolize, which leads to strong background signals and inadequate imaging quality of TAMs nearby the liver such as pancreatic cancer. Herein, we aim to develop metabolizable dextran-indocyanine green (DN-ICG) nanoprobes in the second near-infrared window (NIR-II, 1 000–1 700 nm) for dynamic imaging of TAMs in pancreatic cancer. Compared to free ICG, the NIR-II fluorescence intensity of DN-ICG nanoprobes increased by 279% with significantly improved stability. We demonstrated that DN-ICG nanoprobes could specifically target TAMs through the interaction of dextran with specific ICAM-3-grabbing nonintegrin related 1 (SIGN-R1), which were highly expressed in TAMs. Subsequently, DN-ICG nanoprobes gradually metabolized in the liver yet remained in pancreatic tumor stroma in mouse models, achieving a high signal-to-background ratio (SBR = 7) in deep tissue (∼0.5 cm) NIR-II imaging of TAMs. Moreover, DN-ICG nanoprobes could detect dynamic changes of TAMs induced by low-dose radiotherapy and zoledronic acid. Therefore, the highly biocompatible and biodegradable DN-ICG nanoprobes harbor great potential for precision therapy in pancreatic cancer.
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