RNA提取
核酸
核糖核酸
核酸法
金标准(测试)
严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)
逆转录酶
病毒学
病毒
聚合酶链反应
实时聚合酶链反应
逆转录聚合酶链式反应
萃取(化学)
2019年冠状病毒病(COVID-19)
生物
色谱法
医学
化学
基因
信使核糖核酸
内科学
病理
传染病(医学专业)
疾病
生物化学
作者
Anna Scarabotto,Simona Balestro,Stella Gagliardi,R. Trotti
出处
期刊:Diagnostics
[MDPI AG]
日期:2022-06-27
卷期号:12 (7): 1561-1561
被引量:2
标识
DOI:10.3390/diagnostics12071561
摘要
Background: Rapid diagnosis of COVID-19 is essential in order to restrict the spread of the pandemic, and different approaches for SARS-CoV-2 testing have been proposed as cost-effective and less time-consuming alternatives. For virus detection, the real-time reverse transcriptase–polymerase chain reaction (RT-PCR) technique is still the “gold standard” for accuracy and reliability, but its performance is affected by the efficiency of nucleic acid extraction methods. Objective: In order to improve the SARS-CoV-2 diagnostic workflow, we compared a “standard” commercially available kit, based on viral RNA extraction from human swab samples by magnetic beads, with its technological evolution. The two methods differ mainly in their time consumption (9 vs. 35 min). Methods: We adopted the MAGABIO PLUS VIRUS DNA/RNA PURIFICATION KIT II (BIOER), defined as “standard”, with the automatic extractor BIOER (GenePure Pro fully automatic nucleic acid purification system) to isolate RNA from nasopharyngeal swabs for the detection of SARS-CoV-2 by RT-PCR. We tested this kit with a new faster version of the first one, defined as “rapid” (MAGABIO PLUS VIRUS RNA PURIFICATION KIT II). Results and Conclusion: The two evaluated procedures provided similar analytical results, but the faster method proved to be a more suitable tool for the detection of SARS-CoV-2 from nasopharyngeal swabs, due to a more rapid availability of results, which may contribute to improving both clinical decision making and patient safety.
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