Stepwise design and synthesis of red-emitting, reversible, HOCl chemosensors for monitoring the progression of drug-induced liver injury

detection of HOCl possible. To achieve HOCl detection, we progressively designed three molecules: PTZ, DPTZ, and DPSZ, which successively realized fluorescence imaging of HOCl, extended the emission wavelength, and achieved reversible imaging. Ultimately, DPSZ was successfully applied to living cell imaging, demonstrating the capability for long-term reversible imaging of HOCl and an endoplasmic reticulum (ER) targeting tendency. Furthermore, it successfully monitored the progression of acetaminophen (APAP)-induced liver injury and the therapeutic process mediated by resveratrol. This chemosensor provides an effective tool for the early diagnosis and treatment of DILI, further highlighting the critical role of HOCl in liver injury.