Establishment of a Raman microsphere-based immunochromatographic method for the combined detection of influenza A and B viruses and SARS-CoV-2 antigen on a single T-line

for SARS-CoV-2. The method showed good repeatability for the respiratory viral antigens, with CV values below 15%. Oxymetazoline and commonly used oral medications did not interfere with the test results; the strips did not cross-react with common respiratory virus antigens, demonstrating good specificity. This method does not require any complicated pre-treatment, and all three viruses can be detected simultaneously by titrating one sample, which improves the detection efficiency. The Respiratory Pathogen Multiplex provides a scientific basis for preventing and controlling the spread of respiratory diseases by analyzing data to understand epidemiological trends and the spread of pathogens.