Optimization And Evaluation Of Mouse Models Of Severe Periodontitis

To investigate a more ideal mouse model of severe periodontitis by comparing simple ligature and ligature combined with injection of Porphyromonas gingivalis lipopolysaccharide (P.g. LPS). Compare the methods of constructing a mouse periodontitis model using ligation and ligation with continuous injection of lipopolysaccharide derived from Porphyromonas gingivalis. Before euthanizing the mice 14 days later, the following checks were conducted: body stereomicroscopy to detect the degree of tooth mobility and probing depth; the degree of alveolar bone resorption was analyzed by microcomputed tomography and stereomicroscopy, and the inflammatory level in the blood of the mice was assessed by enzyme-linked immunosorbent assay for interleukin-1β and tumor necrosis factor-α. Compared with the change of tooth mobility and average probing depth in mice with ligation, the changes of tooth mobility and average probing depth were more serious in mice with ligation and continuous injection of P.g. LPS (P<0.05). Compared with percent bone volume, bone mineral density, CEJ-ABC and the area of attachment loss of the left maxillary of mice with ligation, the percent bone volume and bone mineral density of the left maxillary of mice with ligation and continuous injection of P.g. LPS were significantly reduced, CEJ-ABC and attachment loss area were significantly increased (P<0.05). Ligation and continuous injection of P.g. LPS combination model may be more suitable for the study of the development and treatment of severe periodontitis.