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A Combinatorial Approach to Regenerate the Periodontal Ligament and Cementum in a Nondental Microenvironment

牙骨质 牙周纤维 化学 再生(生物学) 牙科 牙囊 牙本质 细胞生物学 医学 干细胞 生物
作者
Yongwen Guo,Mengting He,Peiqi Wang,Ding Bai,Jeong-Hui Park,Khandmaa Dashnyam,Jung Hwan Lee,Olivier Huck,Nadia Benkirane‐Jessel,Hae‐Won Kim,Murugan Ramalingam
出处
期刊:Journal of Tissue Engineering and Regenerative Medicine [Wiley]
卷期号:2023: 1-12
标识
DOI:10.1155/2023/1277760
摘要

While treated dentin matrix (TDM) has been used for regeneration of dental tissues, the quality and quantity of regenerated periodontal tissue structure are suboptimal. The present study was undertaken to test whether the combined use of the TDM with dental follicle cells (DFCs) and Hertwig’s epithelial root sheath (HERS) cells enhances the regeneration of periodontal structures in a nondental microenvironment. TDMs were fabricated from 3-month-old Sprague–Dawley (SD) rats. DFCs and HERS cells were isolated from postnatal 7-day SD rats. Purified DFCs and HERS cells, both in combination or alone, were seeded and cultured on TDM in vitro and characterized. The cell-seeded TDMs were subsequently implanted into a 3-month-old rat greater omentum for 6 weeks, and further histological evaluation was performed. The results showed that cells grew well on the surface of TDMs, and mineralized nodules could be seen, especially in the HERS + DFCs group. After transplantation in rat omentum, periodontal ligament-like fibers and cementum-like structures were observed around the TDM in 1/3 of the samples in both the HERS group and the DFCs group and in 2/3 of the samples in the HERS + DFCs group, while almost no attached tissue formation was found in the TDM only group. The formed cementum width and the periodontal ligament length were significantly larger in the HERS + DFCs group. The periodontal ligament-like fibers in the HERS + DFCs group were orderly arranged and attached to the cementum-like tissues, which resembled the cementum-periodontal structure. Therefore, the combined use of DFCs, TDM, and HERS cells may be a promising strategy for the regeneration of the periodontal structures, especially in the nondental microenvironment.
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