胶原纤维
显微镜
肌腱
圆二色性
光学
污渍
材料科学
二次谐波产生
染色
纤维
曙红
负染色法
H&E染色
二色性
极性(国际关系)
生物物理学
电子显微镜
化学
解剖
病理
结晶学
物理
生物
医学
激光器
生物化学
细胞
作者
MacAulay Harvey,Breanna Lane,Richard Cisek,Samuel P. Veres,Laurent Kreplak,Danielle Tokarz
出处
期刊:Optics Letters
[Optica Publishing Group]
日期:2024-05-29
卷期号:49 (13): 3705-3705
被引量:2
摘要
Circular dichroism second harmonic generation microscopy (CDSHG) is a powerful imaging technique, which allows three-dimensional visualization of collagen fibril orientation in tissues. However, recent publications have obtained contradictory results on whether CDSHG can be used to reveal the relative out-of-plane polarity of collagen fibrils. Here we compare CDSHG images of unstained tendon and tendon which has been stained with hematoxylin and eosin. We find significant differences in the CDSHG between these two conditions, which explain the recent contradictory results within the literature.
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