聚二甲基硅氧烷
硅酮
生物相容性
表面改性
材料科学
生物医学工程
体内
粘附
生物膜
纳米技术
接触角
化学
医学
细菌
复合材料
生物
物理化学
冶金
生物技术
遗传学
作者
Maïssa Dardouri,Ana Bettencourt,Víctor Martín,Filomena A. Carvalho,Bruno Colaço,Adelina Gama,Madeleine Ramstedt,Nuno C. Santos,Maria Helena Fernandes,Pedro Sousa Gomes,Isabel Ribeiro
出处
期刊:Pharmaceutics
[Multidisciplinary Digital Publishing Institute]
日期:2022-08-31
卷期号:14 (9): 1836-1836
被引量:8
标识
DOI:10.3390/pharmaceutics14091836
摘要
Silicone-based medical devices composed of polydimethylsiloxane (PDMS) are widely used all over the human body (e.g., urinary stents and catheters, central venous catheters stents) with extreme clinical success. Nevertheless, their abiotic surfaces, being prone to microorganism colonization, are often involved in infection occurrence. Improving PDMS antimicrobial properties by surface functionalization with biosurfactants to prevent related infections has been the goal of different works, but studies that mimic the clinical use of these novel surfaces are missing. This work aims at the biofunctional assessment of PDMS functionalized with rhamnolipids (RLs), using translational tests that more closely mimic the clinical microenvironment. Rhamnolipids were covalently bonded to PDMS, and the obtained surfaces were characterized by contact angle modification assessment, ATR-FTIR analysis and atomic force microscopy imaging. Moreover, a parallel flow chamber was used to assess the Staphylococcus aureus antibiofilm activity of the obtained surfaces under dynamic conditions, and an in vitro characterization with human dermal fibroblast cells in both direct and indirect characterization assays, along with an in vivo subcutaneous implantation assay in the translational rabbit model, was performed. A 1.2 log reduction in S. aureus biofilm was observed after 24 h under flow dynamic conditions. Additionally, functionalized PDMS lessened cell adhesion upon direct contact, while supporting a cytocompatible profile, within an indirect assay. The adequacy of the biological response was further validated upon in vivo subcutaneous tissue implantation. An important step was taken towards biofunctional assessment of RLs-functionalized PDMS, reinforcing their suitability for medical device usage and infection prevention.
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