谷胱甘肽
荧光
香豆素
检出限
化学
细胞内
荧光寿命成像显微镜
生物物理学
生物化学
生物
色谱法
光学
有机化学
酶
物理
作者
Zhi‐Qiang Hu,Lei‐Li Sun,Yingying Gu,Yi Jiang
标识
DOI:10.1016/j.snb.2015.01.084
摘要
A selective and sensitive method to detect glutathione (GSH) based on the complex of a coumarin-based probe and Cu2+ is reported. In response to GSH, the system provides remarkable fluorescence intensity change with the color change from colorless to yellow, and the detect limit is determined to be 3.6 × 10−7 M. The background amino acids show small or no interference on the detection of GSH. Moreover, this probe could be applied to detect GSH via intracellular fluorescent imaging.
科研通智能强力驱动
Strongly Powered by AbleSci AI