Electronic Absorption Spectra and Redox Properties of C Type Cytochromes in Living Microbes

Shewanella is an electrogenic microbe that has significant content of c type cytochromes (ca. 0.5 mM). This feature allows the optical absorption spectra of the cell-membrane-associated proteins to be monitored in vivo in the course of extracellular respiratory electron-transfer reactions. The results show significant differences to those obtained in vitro with purified proteins.