Electron ionization mass spectrometric fragmentation and multiple reaction monitoring quantification of ferulic and p ‐coumaric acid trimethylsilyl derivatives in deposited atmospheric particles

Rationale Ferulic and p ‐coumaric acids are important biological and structural components of plant cell walls and possess antioxidant and antimicrobial properties. These phenolic acids are widespread in environmental samples. However, when they are present at very low concentrations or in very complex lipid extracts, their identification and quantification can be challenging. Methods The electron ionization mass spectrometry (EI‐MS) fragmentation pathways of ferulic and p ‐coumaric acid trimethylsilyl (TMS) derivatives were investigated. These pathways were deduced by (i) low‐energy collision‐induced dissociation (CID) gas chromatography (GC)/EI‐MS/MS, (ii) accurate mass measurement, and (iii) 13 C labelling. These compounds were then characterized and quantified in multiple reaction monitoring (MRM) mode in total lipid extracts of deposited atmospheric particles using highly specific transitions based on the main fragmentation pathways elucidated. Results Low‐energy CID‐MS/MS analyses, accurate mass measurement and 13 C labelling enabled us to elucidate EI‐MS fragmentations of ferulic and p ‐coumaric acid TMS derivatives. Some specific fragmentations proved useful for subsequent characterization and quantification of these compounds. As an application of some of the described fragmentations, trace amounts of these phenolic acids were characterized and quantified in MRM mode in wet‐ and dry‐deposited atmospheric particles containing low proportions of organic matter. Conclusions EI‐MS fragmentations of ferulic and p ‐coumaric acid TMS derivatives exhibit specific fragment ions that can be very useful for the quantification of trace amounts of both phenolic acids in environmental samples.