化学
天竺葵苷
氰化物
邻苯三酚
飞燕草素
花青素
儿茶酚
马维定
欧洲越橘
芍药苷
吸光度
哌替尼丁
部分
色谱法
儿茶素
核化学
作者
Francesca Torrini,Lapo Renai,Simona Scarano,M. Del Bubba,Pasquale Palladino,Maria Minunni
出处
期刊:Talanta
[Elsevier]
日期:2021-12-01
卷期号:240: 123156-123156
被引量:1
标识
DOI:10.1016/j.talanta.2021.123156
摘要
Here is examined the colour development from common anthocyanins (i.e., cyanidin, delphinidin, malvidin, and pelargonidin glycosides) and from anthocyanins-rich extracts (i.e., bilberries, strawberries, and raspberries), using zinc-anthocyanin complexes as molecular probe. We have observed the absorbance increase in the blue region in presence of large excess of zinc ion at acidic pH for cyanidin and delphinidin derivatives, likely due to quinoidal base stabilization from catechol and pyrogallol moiety. The assay condition were studied and applied to natural extracts containing these compounds. The same behaviour was observed for bilberry and, to a minor extent, for raspberry extracts, due to the larger cyanidin/delphinidin contents in the former than in the latter. Anthocyanin standard UV-Vis analysis in buffer has shown a very good linear correlation for cyanidin and delphinidin (R2 = 0.995 and 0.997, respectively), good precision (CV% = 7.4% and 5.3% respectively), high sensitivity (Cyε600nm = 8300 M-1 cm-1, LOD = 0.264 ± 0.005 mg L-1, LOQ = 0.478 ± 0.007 mg L-1, and Dpε600nm = 15,900 M-1 cm-1, LOD = 0.143 ± 0.002 mg L-1, LOQ = 0.478 ± 0.007 mg L-1). The effectiveness of this colorimetric method for the selective quantification of catechol/pyrogallol-based anthocyanins has been demonstrated in the aforementioned complex real matrices and compared to LC-MS/MS analysis and pH-differential method, offering a valuable tool to characterize plant and food extracts particularly rich in zinc-coordinating anthocyanins.
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