Chiral gold clusters functionalized two-dimensional nanoparticle films to regulate the adhesion and differentiation of stem cells

Chirality has been proved to play a significant role in tuning cell behaviors and controlling cellular functions. Up to now, almost all the chirality origins of extracellular microenvironment are belong to chiral ligands induction or direct chiral patterns. In this study, chiral gold nanoclusters (L/D-AuNC) loaded on two-dimensional gold nanoparticle films (L/D-film) with multiple chirality origins were prepared to regulate the adhesion and differentiation of mouse bone marrow mesenchymal stem cells (MSCs). MSCs on the D-film exhibited higher cell density and larger spreading area, and more cells differentiated into osteoblasts. Compared with D-film, L-film has a lower cell density and smaller spreading area, and more adipoblasts are achieved. The corresponding expression results of osteogenic differentiation marker (RUNX2) also confirmed the above experimental phenomenon. These results demonstrated that the chirality of clusters has great effect on the direction of cell fate.