SH-SY5Y型
细胞凋亡
细胞周期
信号转导
草铵膦
生物
活力测定
细胞生物学
Wnt信号通路
毒性
程序性细胞死亡
细胞毒性
生物化学
化学
细胞培养
遗传学
草甘膦
生物技术
体外
有机化学
神经母细胞瘤
作者
Yin Zhu,Yongan Xu,Yaoyao Dai,Guizhen Zhang,Chenyang Ji,Quan Zhang,Meirong Zhao
标识
DOI:10.1016/j.scitotenv.2023.165106
摘要
Glufosinate (Glu), a broad-spectrum and highly effective non-selective herbicide, behaves in typical chiral features to target organisms. However, the information on the enantioselective toxicity of DL-Glu and L-Glu against non-target organisms is still limited especially at environmental concentrations. In this study, we investigated the potential mechanism accounting for the enantioselective cytotoxicity of Glu based on cell cycle and apoptosis. Results showed that DL-Glu and L-Glu had no suppression on cell viability at 10−5 M, however, SH-SY5Y cells were significantly arrested at G1/G0 phase after L-Glu exposure compared with DL-Glu. The apoptosis assay exhibited an increase in late apoptosis cells and a decrease in viable cells for DL-Glu and L-Glu treatment. The bioinformatics analysis demonstrated that alterations in transcription translation and signal transduction including “calcium signaling pathway”, “Wnt signaling pathway”, “FoxO signaling pathway” were the possible pathways responsible for Glu-induced enantioselectivity in cell cycle and apoptosis. Interestingly, the Gene Set Enrichment Analysis (GSEA) also revealed the probable association between DL-Glu exposure and degenerative diseases. These findings serve as a reminder that caution should be exercised not only when using pesticide racemates but also when promoting or applying single- or enriched-isomer pesticides.
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