Study on chitosan/gelatin hydrogels containing ceria nanoparticles for promoting the healing of diabetic wound

Abstract Chronic inflammation at diabetic wound sites results in the uncontrolled accumulation of pro‐inflammatory factors and reactive oxygen species (ROS), which impedes cell proliferation and delays wound healing. To promote the healing of diabetic wounds, chitosan/gelatin hydrogels containing ceria nanoparticles (CNPs) of various sizes were created in the current study. CNPs' efficacy in removing , •OH, and H 2 O 2 was demonstrated, and the scavenging ability of CNPs of varying sizes was compared. The in vitro experiments demonstrated that hydrogels containing CNPs could effectively protect cells from ROS‐induced damage and facilitate mouse fibroblast migration. Furthermore, during the treatment of diabetic wounds in vivo, hydrogels containing CNPs exhibited anti‐inflammatory activity and could reduce the expression of the pro‐inflammatory factors TNF‐α (above 30%), IL‐6 (above 90%), and IL‐1β (above 80%), and effectively promote wound closure (above 80%) by inducing re‐epithelialization, collagen deposition, and angiogenesis. In addition, the biological properties and therapeutic effects of hydrogels containing CNPs of various sizes were compared and discussed. The finding revealed that hydrogels with 4 nm CNPs exhibited more significant biological properties and had implications for diabetic wound treatment.