ANALYSIS OF β-SITOSTEROL IN SUPPLEMENT USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY: DEVELOPMENT AND VALIDATION

β-sitosterol is one phytosterol commonly found in plant membranes. It has been reported to have many potential benefits such as antioxidant, antimicrobial, antiviral, antidiabetic, anticancer, anti-inflammatory, and antipyretic activities. Various nutraceutical supplements containing β-sitosterol are marketed worldwide. The claim regarding health benefits is used on the label of the supplement, so quality control is needed to ensure the safety, quality, and efficacy of these products. Hence, this study aimed to develop and validate a simple and reliable HPLC method for the determination of β-sitosterol in supplements. The method utilizes an LC Column 150 x 4.6 mm Phenomenex with isocratic elution of methanol and acetonitrile (90: 10 v/v). An effluent flow rate of 1.5 mL/min and UV detection at 202 nm was used for the analysis of β-sitosterol. A validated HPLC method has been developed and used for the quantification of β-sitosterol. The developed method has high precision and accuracy, with a rapid sample preparation procedure and short analysis time, which is suitable for quality control of β-sitosterol in various supplements.