循环肿瘤细胞
声表面波
单元格排序
化学
分离(微生物学)
分类
微流控
聚苯乙烯
炸薯条
纳米技术
色谱法
细胞
材料科学
聚合物
癌症
声学
生物化学
计算机科学
生物信息学
物理
生物
医学
有机化学
内科学
程序设计语言
转移
工程类
电气工程
作者
Wenping Geng,Yukai Liu,Nanxin Yu,Xiaojun Qiao,Miaomiao Ji,Yaokai Niu,Liya Niu,Wenxiao Fu,Huiyi Zhang,Kaixi Bi,Xiujian Chou
标识
DOI:10.1016/j.aca.2023.341138
摘要
Obtaining highly purified intact living cells from complex environments has been a challenge, such as the isolation of circulating tumor cells (CTCs) from blood. In this work, we demonstrated an acoustic-based ultra-compact device for cell sorting, with a chip size of less than 2 × 1.5 cm2. This single actuator device allows non-invasive and label-free isolation of living cells, offering greater flexibility and applicability. The device performance was optimized with different-sized polystyrene (PS) particles and blood cells spiked with cancer cells. Using the narrow-path travelling surface acoustic wave (np-TSAW), precise isolation of 10 μm particles from a complex mixture of particles (5, 10, 20 μm) and separation of 8 μm and 10 μm particles was achieved. The purified collection of 10 μm particles with high separation efficiency (98.75%) and high purity (98.1%) was achieved by optimizing the input voltage. Further, we investigated the isolation and purification of CTCs (MCF-7, human breast cancer cells) from blood cells with isolation efficiency exceeding 98% and purity reaching 93%. Viabilities of the CTCs harvested from target-outlet were all higher than 97% after culturing for 24, 48, and 72 h, showing good proliferation ability. This novel ultra-miniaturized microfluidic chip demonstrates the ability to sorting cells with high-purity and label-free, providing an attractive miniaturized system alternative to traditional sorting methods.
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