From 2017 through 2021, the local participation of voluntary non-remunerated blood donation showed an overall increasing trend, and the HIV infection prevalence maintained relatively stable in the blood donating population, with the infected individuals being dominated by sexually active young male office employees and students. There existed certain differences in the screening efficacies of distinct HIV ELISA reagents. The au-thorized laboratories should comprehensively evaluate the selection of blood screening programs and reagents to effectively conserve blood resources and reduce the risk of blood-borne HIV transmission.