蛋白酵素
黑腹果蝇
舍宾
丝氨酸蛋白酶
先天免疫系统
生物
丝氨酸
蛋白酶
细胞生物学
免疫系统
抗菌肽
生物化学
基因
遗传学
酶
肽
作者
Tisheng Shan,Yan Wang,Krishna Bhattarai,Haobo Jiang
出处
期刊:Science Advances
[American Association for the Advancement of Science (AAAS)]
日期:2023-12-22
卷期号:9 (51)
被引量:2
标识
DOI:10.1126/sciadv.adk2756
摘要
Melanization and Toll pathway activation are essential innate immune mechanisms in insects, which result in the generation of reactive compounds and antimicrobial peptides, respectively, to kill pathogens. These two processes are mediated by phenoloxidase (PO) and Spätzle (Spz) through an extracellular network of serine proteases. While some proteases have been identified in Drosophila melanogaster in genetic studies, the exact order of proteolytic activation events remains controversial. Here, we reconstituted the serine protease framework in Drosophila by biochemical methods. This system comprises 10 proteases, i.e., ModSP, cSP48, Grass, Psh, Hayan-PA, Hayan-PB, Sp7, MP1, SPE and Ser7, which form cascade pathways that recognize microbial molecular patterns and virulence factors, and generate PO1, PO2, and Spz from their precursors. Furthermore, the serpin Necrotic negatively regulates the immune response progression by inhibiting ModSP and Grass. The biochemical approach, when combined with genetic analysis, is crucial for addressing problems that long stand in this important research field.
科研通智能强力驱动
Strongly Powered by AbleSci AI