上睑下垂
石英晶体微天平
材料科学
赫拉
细胞凋亡
程序性细胞死亡
生物物理学
细胞
化学
生物化学
生物
吸附
有机化学
作者
Wenwei Li,Jing Li,Yanyang Wu,Tiean Zhou
出处
期刊:Biosensors
[Multidisciplinary Digital Publishing Institute]
日期:2023-03-07
卷期号:13 (3): 356-356
被引量:7
摘要
In this study, a double resonator piezoelectric cytometry (DRPC) technology based on quartz crystal microbalance (QCM) was first employed to identify HeLa cell pyroptosis and apoptosis by monitoring cells’ mechanical properties in a real-time and non-invasive manner. AT and BT cut quartz crystals with the same frequency and surface conditions were used concurrently to quantify the cells-exerted surface stress (ΔS). It is the first time that cells-exerted surface stress (ΔS) and cell viscoelasticity have been monitored simultaneously during pyroptosis and apoptosis. The results showed that HeLa pyroptotic cells exerted a tensile stress on quartz crystal along with an increase in the elastic modulus (G′), viscous modulus (G″), and a decrease of the loss tangent (G″/G′), whereas apoptotic cells exerted increasing compressive stress on quartz crystal along with a decrease in G′, G″ and an increase in G″/G′. Furthermore, engineered GSDMD−/−-DEVD- HeLa cells were used to investigate drug-induced disturbance and testify the mechanical responses during the processes of pyroptosis and non-pyroptosis. These findings demonstrated that the DRPC technology can serve as a precise cytomechanical sensor capable of identifying pyroptosis and apoptosis, providing a novel method in cell death detection and paving the road for pyroptosis and apoptosis related drug evaluation and screening.
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