Detection of Red-Eared Slider (Trachemys scripta elegans) using Environmental DNA with Cytochrome b Primer

Abstract Biomonitoring surveys for the presence of invasive species need effective and efficient methods. Molecular methods based on the detection of genetic material in environmental samples or environmental DNA (eDNA) provide better resolution for detection, especially in elusive species. The eDNA-based method is an approach that has been widely used for conservation and business management because it is a non-invasive method. Cytochrome b primer is a specific primer that can be used to amplify Red-Eared Slider eDNA from water samples, eDNA was amplified using qPCR. The results obtained after performing qPCR are in the form of Ct values that can be used to estimate the biomass of Red-Eared Slider.