乳状液
化学
化学工程
油菜籽
流变学
吸附
油滴
色谱法
蛋白质聚集
同种类的
基质(化学分析)
材料科学
有机化学
生物化学
食品科学
复合材料
工程类
物理
热力学
作者
Eleni Ntone,Remco Kornet,Paul Venema,M.B.J. Meinders,Erik van der Linden,Johannes H. Bitter,Leonard M.C. Sagis,Constantinos V. Nikiforidis
标识
DOI:10.1016/j.foodhyd.2021.107400
摘要
We investigated the complementary roles of napins and cruciferins present in a rapeseed protein mixture (RPM) in structuring emulsion-filled gels (EFGs). Napins are small molecular weight albumins with high interfacial activity, while cruciferins are high molecular weight globulins that form gels upon heat-induced gelation. The role of napins is to stabilize the emulsion droplets, while cruciferins, which were previously found to interact with the adsorbed napin interfacial layer, can build the protein gel network around the droplets. The effects of oil concentration (0–30 wt%) and pH (5 and 7) on the rheological and microstructural properties of EFGs were investigated. In the absence of oil, at pH 5, due to low protein solubility, RPM formed a heterogeneous network built of protein aggregates. At pH 7, RPM was more soluble and formed a homogeneous network built of strand-like protein structures with higher gel firmness. In the presence of emulsion droplets, the gel firmness increased, with a more pronounced reinforcement at pH 5 compared to pH 7. The type of gel network did not change by the presence of emulsion droplets neither at pH 5 nor at pH 7, as suggested from confocal microscopy and the unchanged response to large deformation. This implies that oil did not really change the protein network structure. The emulsion droplets, being stiffer than the protein matrix, and being integrated in the structural matrix, increased the gel firmness. This research shows that the presence of two different proteins with complementary roles in a less purified protein extract, provides a single protein ingredient suitable for structuring food emulsion-filled gels.
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