克伦特罗
明胶
色谱法
校准曲线
化学
分析物
检出限
质谱法
高效液相色谱法
作者
Benjamín Velasco-Bejarano,Anuar Gómez-Tagle,Maria Olivia Noguez-Córdova,María L. Zambrano-Zaragoza,Alfonso Miranda-Molina,Jahir Bautista,Leonardo Rodríguez,Ricardo Velasco-Carrillo
标识
DOI:10.1016/j.foodchem.2021.131261
摘要
Clenbuterol is present in animal tissues and organs and, therefore, potentially present in gelatin derived from animal sources. The objective of this study was to develop a method for identify an quantify traces of clenbuterol in gelatin and jellies. The clenbuterol calibration curve showed linearity in the range of 20-1000 pg mL-1. The detection and quantification limits were 5 pg g-1 and 10 pg g-1, respectively. The recovery of the analyte ranged from 93.4 to 98.7% with an intra-day RSD% (n = 4) of 1.25%-3.25%, and an inter-day RSD% (n = 12) of 0.5%-2.25%, with good linearity (R2 = 0.99). The method developed and validated was successfully applied in 54 gelatin samples, 57.4% of which showed clenbuterol. This UHPLC-MS/MS method combines high sensitivity with good selectivity and short chromatographic run time.
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