Structural characterization of polysaccharide from yellow sweet potato and ameliorates DSS-induced mice colitis by active GPR41/MEK/ERK 1/2 signaling pathway

A polysaccharide isolated from yellow sweet potato (Ipomoea batatas (L.) Lam.) consisted of Rha, Ara, Gal, Glc, GalA, GlcA with the ratio of 1.00, 2.00, 3.63, 1.21, 1.17, 1.14, respectively. The molecular weight (Mw) of RSPP-A was determinted to be 2.51×106 kDa. Methylation, Nuclear Magnetic Resonance (NMR) (1D & 2D) and Fourier transform infrared spectroscopy (FT-IR) analysis indicated that RSPP-A possessed six glycosidic bonds including α-L-Araf-(1→, →5)-α-L-Araf-(1→, →6)-β-D-Galp-(1→, β-D-Glcp-(1→, →3)-α-L-Araf-(1→, →3)-α-L-Rhap-(1→. In dextran sulfate sodium (DSS) induced mouse-acute-colitis model, the results indicated that RSPP-A could down- regulate the secretion of IL-6 and IL-1β, and promote the secretion of IL-10 in serum and colon, which also suggested that RSPP-A could enhance the contents of short chain fatty acids(SCFAs) and up-regulate the expression of G protein-coupled receptor (GPR41) in colon. Moreover, the expression of Mitogen-activated protein kinase kinase (MEK), extracellular signal-regulated kinase 1/2 (ERK1/2) were up-regulated in colon after intervention with RSPP-A, result from above suggested that the anti-inflammatory activity might be related to the production of SCFA, activating GPR41/MEK/ERK1/2 signaling pathway.