小RNA
生物
多重耐药
基因表达谱
抗药性
小桶
基因
基因表达
计算生物学
癌症
折叠变化
生物信息学
癌症研究
遗传学
转录组
作者
Zhangqian Chen,Lin Zhang,Limin Xia,Yanling Jin,Qing Wu,Hao Guo,Xin Shang,Jun Dou,Kaichun Wu,Yongzhan Nie,Daiming Fan
出处
期刊:Cancer Letters
[Elsevier]
日期:2014-08-01
卷期号:350 (1-2): 43-51
被引量:24
标识
DOI:10.1016/j.canlet.2014.04.010
摘要
Understanding the mechanism underlying multidrug resistance and identifying effective targets that can overcome it is of critical importance. In this study, mRNA and miRNA expression profiling of the drug resistant sublines, SGC7901/VCR and SGC7901/ADR, and their parental gastric cancer cell line SGC7901 were performed. A significant number of genes and a limited subset of miRNAs were commonly dysregulated, which were further validated using qRT-PCR. GO and KEGG pathway analyses of the commonly dysregulated genes indicated that the MAPK signalling pathway may be involved in multidrug resistance, which was further validated using immunoblotting and MTT assay. Finally a primary multidrug resistance network in gastric cancer, consisting of the commonly dysregulated genes and miRNAs, was established and functional miRNA-mRNA pairs were identified. The commonly dysregulated genes and miRNAs identified in this study may represent good therapeutic targets and further study of these targets may increase our understanding of the mechanisms underlying multidrug resistance.
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