甾醇
角鲨烯单加氧酶
突变体
活性氧
角鲨烯
拟南芥
生物化学
生物
氧化酶试验
拟南芥
NADPH氧化酶
生物合成
细胞生物学
植物
化学
酶
基因
胆固醇
作者
David Posé,Itziar Castanedo,Omar Borsani,Benjamín Nieto,Abel Rosado,Ludivine Taconnat,Albert Ferrer,Liam Dolan,Victoriano Valpuesta,Miguel A. Botella
出处
期刊:Plant Journal
[Wiley]
日期:2009-06-25
卷期号:59 (1): 63-76
被引量:119
标识
DOI:10.1111/j.1365-313x.2009.03849.x
摘要
Squalene epoxidase enzymes catalyse the conversion of squalene into 2,3-oxidosqualene, the precursor of cyclic triterpenoids. Here we report that the Arabidopsis drought hypersensitive/squalene epoxidase 1-5 (dry2/sqe1-5) mutant, identified by its extreme hypersensitivity to drought stress, has altered stomatal responses and root defects because of a point mutation in the SQUALENE EPOXIDASE 1 (SQE1) gene. GC-MS analysis indicated that the dry2/sqe1-5 mutant has altered sterol composition in roots but wild-type sterol composition in shoots, indicating an essential role for SQE1 in root sterol biosynthesis. Importantly, the stomatal and root defects of the dry2/sqe1-5 mutant are associated with altered production of reactive oxygen species. As RHD2 NADPH oxidase is de-localized in dry2/sqe1-5 root hairs, we propose that sterols play an essential role in the localization of NADPH oxidases required for regulation of reactive oxygen species, stomatal responses and drought tolerance.
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