脱氧核酶
自催化
化学
小RNA
体内
生物物理学
临床前影像学
DNA
纳米技术
生物化学
材料科学
生物
遗传学
催化作用
基因
作者
Jie Wei,Huimin Wang,Qiong Wu,Xue Gong,Ke Ma,Xiaoqing Liu,Fuan Wang
标识
DOI:10.1002/anie.201911712
摘要
Abstract DNAzymes have been recognized as promising transducing agents for visualizing endogenous biomarkers, but their inefficient intracellular delivery and limited amplification capacity (including insufficient cofactor supply) preclude their extensive biological application. Herein, an autocatalytic DNAzyme (ACD) biocircuit is constructed for amplified microRNA imaging in vivo based on a hybridization chain reaction (HCR) and DNAzyme biocatalysis, sustained by a honeycomb MnO 2 nanosponge (hMNS). The hMNS not only delivers DNA probes, but also supplies Mn 2+ as a DNAzyme cofactor and magnetic resonance imaging (MRI) agent. Through the subsequent cross‐activation of HCR and DNAzyme amplicons, the ACD amplifies the limited signal resulting from miRNA recognition. The hMNS/ACD system was used to image microRNA in vivo, thus demonstrating its great promise in cancer diagnosis.
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