2-benzothiazoleacetonitrile based two-photon fluorescent probe for hydrazine and its bio-imaging and environmental applications

Abstract A novel turn-on two-photon fluorescent probe NS-N 2 H 4 was developed with the 2-benzothiazoleacetonitrile as a new recognition site for the detection of hydrazine (N 2 H 4 ). The two-photon probe exhibited favorable properties including high selectivity, low cytotoxicity and almost 16-fold fluorescence enhancement in the presence of N 2 H 4 in solution. The probe could be used to image hydrazine in the living cells. Notably, we also used the two-photon fluorescent probe to image hydrazine in the tissue imaging for the first time. Furthermore, by the way of probe-loaded TLC plate, we further monitored vapor of hydrazine. Therefore, the novel two-photon probe is expected to be employed to detect N 2 H 4 in biosamples and environmental pollution and the new recognition site will be widely applied to construct fluorescent probes for the detection of N 2 H 4 .