An efficient condition of Saponification of Lutein ester from marigold flower

Saponification of carotenoid esters leads to decomposition at high temperature and high concentration of alkali. Lutein ester is collected from marigold flower. The saponification of purified lutein ester is studied at different parameters using UV-visible spectrophotometer, HPLC. Findings showed efficient saponification in 0.5 M KOH at 500C for 30minutes.