电穿孔
生物技术
纳米技术
生物
计算生物学
生化工程
遗传学
材料科学
工程类
基因
作者
Tadej Kotnik,Wolfgang Frey,Martin Sack,Saša Haberl Meglič,Matjaž Peterka,Damijan Miklavčič
标识
DOI:10.1016/j.tibtech.2015.06.002
摘要
Electroporation is already an established technique in several areas of medicine, but many of its biotechnological applications have only started to emerge; we review here some of the most promising. We outline electroporation as a phenomenon and then proceed to applications, first outlining the best established - the use of reversible electroporation for heritable genetic modification of microorganisms (electrotransformation), and then explore recent advances in applying electroporation for inactivation of microorganisms, extraction of biomolecules, and fast drying of biomass. Although these applications often aim to upscale to the industrial and/or clinical level, we also outline some important chip-scale applications of electroporation. We conclude our review with a discussion of the main challenges and future perspectives.
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