Various Culture Media Effect on T4 Phage Lysis and Production

Studies on bacteriophage growth and its development played a vital role in the history of molecular biology which in turn helped in clarification of many points. Most of the previous studies on bacteriophage development and growth have been performed under optimal conditions for the host cell. While On the other hand these conditions may not be optimal for the T4 bacteriophage. As a matter of fact in nature E. coli faces many unfavorable growth conditions, good example are those conditions prevailing in the human gut in which E.coli manages to survive well. This study characterizes the effects and influences of well-defined physiological conditions on T4 bacteriophage growth and development. In addition to this, T4 bacteriophage interactions with its bacterial host have also been demonstrated.In our present study we observed that the maximum growth and lysis of T4 bacteriophage was on luria-bertani (LB) and nutrient media (NM). Moreover the T4 bacteriophage production and lysis was also good in luria-bertani plus glucose (LB+G) media but when compared with its production in luria-bertani (LB) and nutrient media it was found to be less than these medium. Our study results also showed that in minimal media (MM) rate of growth and lysis activity of T4 bacteriophage was lowest as compared to other mentioned medium.