Cover Feature: Switching the Cofactor Specificity of an Imine Reductase (ChemCatChem 1/2018)

The Cover Feature shows the switch of the cofactor specificity of an imine reductase from NADPH to NADH. In their Full Paper, N. Borlinghaus and B. M. Nestl demonstrate that by applying the CSR-SALAD, a tool for engineering enzymatic nicotinamide cofactor preference, and enlarging the mutant library by further amino acid substitutions, the NAPDH coenzyme preference of the imine reductase from Myxococcus stipitatus can be engineered. The mutagenesis of the nicotinamide binding pocket resulted in variants with reversed specificity and recovered activity. More information can be found in the Full Paper by N. Borlinghaus and B. M. Nestl on page 183 in Issue 1, 2018 (DOI: 10.1002/cctc.201701194).