体内
检出限
荧光
癌细胞
体外
癌症
材料科学
生物物理学
适体
线性范围
纳米技术
离体
介孔二氧化硅
化学
介孔材料
分子生物学
色谱法
生物
生物化学
物理
遗传学
生物技术
催化作用
量子力学
作者
Chengyi Li,Ying Meng,Shanshan Wang,Min Qian,Jianxin Wang,Weiyue Lu,Rongqin Huang
出处
期刊:ACS Nano
[American Chemical Society]
日期:2015-11-20
卷期号:9 (12): 12096-12103
被引量:87
标识
DOI:10.1021/acsnano.5b05137
摘要
Multiple diagnosis of cancer by a facile fluorescent sensor is extremely attractive. Herein, a Cy3-labeled ssDNA probe (P0-Cy3) was π-π stacked on the surface of oxidized mesoporous carbon nanospheres (OMCN) to construct the fluorescent "turn-on" aptasensor. Attributing to the intrinsic properties of OMCN, the OMCN-based aptasensor not only can be used to detect mucin1 protein in liquid with a wide range of 0.1-10.6 μmol/L, a low detection limit of 6.52 nmol/L, and good selectivity, but also can quantify the cancer cells in solution with the linear range of 10(4)-2 × 10(6) cells/mL and a detection limit of 8500 cells/mL. Fascinatingly, this OMCN-based aptasensor was exploited to image cancer via solid tissues such as cells, tissue sections, and ex vivo and in vivo tumors, in which the obvious distinguishability between cancer and normal tissues was clearly demonstrated. This is a robust and simple detection technique, which can well achieve the multiple diagnosis of cancer in vitro and in vivo.
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