A lateral flow immunoassay "Sss AgriStrip" as a tool for specific and rapid detection of Spongospora subterranea on potato tubers

Results of the Sss AgriStrip were highly consistent with DAS-ELISA, PCR, real-time PCR, and microscopy. Sss was detected in all tubers with typical symptoms but only in a few lots with tuber s showing atypical (suspicious ) lesions. The appearance of these atypical lesions positive for the presence of Sss was similar: a diff use brown necrotic tissue under the periderm and the absence of the dark brown Sss sporeballs (photo lot 5b) . Sss was not detected in most of the atypical lesions with the Sss AgriStrip as well as with the other methods. The tubers with the atypical lesions free of Sss were analyzed for the presence of Streptomyces . Bands start developing after 1-2 min and reach maximum intensity after 10-15 min.