Parthenogenetic Activation on Porcine Oocytes by Ethanol Stimulation and Electrical Stimulation

The matured porcine oocytes were activated by ethanol stimulation and electrical stimulation.The results showed that the cleavage of electrical stimulation with two 1.6 kV/cm and 60 μs was higher(88.68%,94/106),and the cleavage rate of ethanol stimulation with 8% and 10 min was 84.21%(64/76).The method of electrical stimulation with two 1.6 kV/cm and 60 μs had higher cleavage rate,and laid the foundation for further study.