Cloning and Sequence Analysis of SCF Gene in Goat Skin Tissue

To investigate the structure and sequence of SCF gene in goat, primers were designed based on the sequence of goat and sheep. Two transcript splices were obtained with PCR and cloning and bioinformatics analysis of the sequence was made. The results showed that the length of coding sequence of soluble and transmembrane SCF gene in goat is 825 bp and 741 bp, respectively. The length difference was due to the deletion / insertion of 6th exon. The identity of SCF gene between goat and sheep or cattle was 98% and 97%, respectively. The phylogenetic clusters based on SCF gene sequence of thirteen species were the same as the zootaxy.