Determination of Organic Acid Compounds in Schisandra Processed with Vinegar

Objective:To established a HPLC method for determination of three organic acid compounds in Schisandra processed with vinegar.Method: High performance liquid chromatography was performed on content determination of three organic acid compounds.Acetonitrile-15 mmol·L-1 KH2PO4(1∶99) were used as the mobile phase and the detected wavelength were at 210 nm for quinic acid and citric acid determination;the mobile phase of protocatechuic acid were methyl-0.5% glacial acid(25∶75) and detect wavelength was set at 260 nm.Result: Three organic acid compounds can be detected in different samples,and the average content of the total amount was about 17.6%,among which the citric acid was highest for almost 15.6%.Conclusion: As an important part of material basis of Schisandra pieces,we should take the organic acids as the quality evaluation index along with the lignans and carry out further research on pharmacological in different pieces of Schisandra.It will provide scientific basis for processing principle research of Schisandra pieces.