Mixotrophic and heterotrophic growth of Haematococcus pluvialis and the request of carbon resource were investigated. The microalga grew better on acetate than glucose and other carbons in both growth modes. The optimum growth concentration of acetate in mixotrophic and heterophic were 0.5- l.0g / L and l.0-l.5g/ L respectively. The average growth rates of Anxotrophjc and heterophic were 0.72d~-1 and 0.53d-1 respectively. After cultured for 8 days, the cell dry weight of mixotrophic and heterotrophic were 0.65g / L and 0.32g / L respectively. Compared with phototrophic (control), the average growth rate and cell dry weight of mixotrophic increased, but in heterotrophic, they decreased.