Effects of Timosaponin on Absorption of Mangiferin

Objective To investigate the effects of timosaponin on absorption of mangiferin by in-vivo and in-vitro methods.Methods LC/MS/MS method was used to determine plasma mangiferin concentration in rats after intragastric administration of mangiferin or mangiferin mixed with timosaponin,and the main pharmacokinetic parameters were calculated.Intestinal absorption of mangiferin was studied by using everted gut sacs and by UPLC.Results Compared with mangiferin group,the plasma concentration,AUC and Cmax of mangiferin were significantly increased,and Tmax was prolonged to 3 h in mangiferin-timosaponin group.Q90,Vt and Paap of mangiferin at ileac segment of rats in timosaponin-mangiferin(1∶1) group were higher than that in mangiferin monomer group(P 0.05).Conclusion Timosaponin can increase the absorption of mangiferin.