Comparative analysis of cordycepin and adenosine contents in fermentation supernatants between Aspergillus nidulans and Cordyceps militaris

The cordycepin and adenosine contents in fermentation supernatants of Cordyceps militaris and Aspergillus nidulans were detected by HPLC,and the effects of different fermentation conditions on the cordycepin and adenosine contents were studied systematically.The results showed that cordycepin and adenosine could be well detected by clearly separated HPLC peaks in proper experimental conditions,and the two components had a good linear relationship and a satisfied recovery rate.The retention time of cordycepin and adenosine peaks was 11.443 min and 9.054 min respectively.The maximum cordycepin and adenosine productions were 249.46 μg/mL and 46.98 μg/mL respectively under the optimal fermentation conditions for Aspergillus nidulans,and the highest cordycepin and adenosine productions were 228.45 μg/mL and 16.95 μg/mL respectively for Cordyceps militaris.Therefore,as a new source of extracting cordycepin and adenosine,Aspergillus nidulans was better or much better than Cordyceps militaris.