Studies on the chemical constituents of the fermentation liquid from marine actinomyces Micromonospora sp.and bacteria Oceanospirillum sp.

Objective To study the chemical constituents of the fermentation liquid from marine actinomyces Micromonospora sp.and bacteria Oceanospirillum sp.Methods The compounds were separated by column chromatography and their structures were elucidated based on chemical and spectral analyses.Result Five compounds were isolated from the ethyl acetate and n-butanol fractions of the fermentation liquid from Micromonospora sp..Their structures were determined as dibutylphthalate(Ⅰ);phthalic acid bis-(2-ethyl-hexyl)ester(Ⅱ);ergosta-5,7,22-triene-3β-ol(Ⅲ);thymine(Ⅳ);amber acid(Ⅴ),respectively.Six compounds were isolated from the ethyl acetate and n-butanol fractions of the fermentation liquid from Oceanospirillum sp..Their structures were determined as 3-isopropyl-pyrrolopiperazine-2,5-dione(Ⅵ);5α,8α-epidioxyergosta-6,22-dien-3β-ol(Ⅶ);pyrrolopiperazine-2,5-dione(Ⅷ);thymine(Ⅳ);uracil(Ⅸ);mannitol(Ⅹ),respectively.Conclusion Compounds Ⅰand Ⅱwere isolated from this genus for the first time.