Study on the clinical significance of serum TNF-α,IL-6,IL-8 concentration and T cell subsets in children patients with mycoplasma pneumonia

Objective To investigate the changes of concentration tumor necrosis factor-a(TNF-a),interleukik-6(IL-6),interleukik-8(IL-8)and peripheral blood T cell subsets(CD+3/CD+4/CD+8)in children patients with mycoplasma pneumonia.Methods 25 normal children were selected as the control group,50 cases of MPP were divided into two groups which 25 cases were common mycoplasma pneumonia(experimental group A) and 25 cases were severe mycoplasma pneumonia(experimental group B).Enzyme linked immunoassay(ELISA)was used to measure serum TNF-α,IL-6,IL-8 concentration in the children.Peripheral blood T cell subsets(CD+3/CD+4/CD+8) in children were determined with flow eytometry.Results The levels of serum TNF-α,IL-6,IL-8 in experimental group A and B were significant higher than those in control group,P0.05,and those in experimental group B were significant higher than those in experimental group A,P0.05.The CD+3,CD+3CD+4 percentage and CD+4/CD+8 in patients with MPP were significantly lower than those in control group,P0.05,and those in experimental group B were significant lower than those in experimental group A,P0.05.Conclusions The levels of TNF-α,IL-6,IL-8 were positively correlated with MPP severity,but the changes of CD+3,CD+3CD+4 percentage and CD+4/CD+8 were negatively correlated with MPP severity.