Enzymatic Process of Ethanol Extract from Periplaneta americana by Papain

Objective:To study on enzymatic technology of ethanol extract from Periplaneta americana by papain and screen out optimum enzymatic technology.Method: Taking degree of hydrolysis as index,effect of enzymatic temperature,enzymatic time,pH,the amount of papain and substrate concentration on papain were investigated by single factor experiment and orthogonal test.Result: Optimum enzymatic technology conditions of papain was determined as follows:enzymatic temperature 55 ℃,enzymatic time 3 h,pH 7.0,the amount of papain 8 400 U·mg-1,substrate concentration 0.5%.Under these conditions,degree of hydrolysis could reach to 9.87%.Molecular weight of sample were smaller and molecular weight distribution were wider after enzymolysis.Conclusion: This optimized method was stable,easy to operate,and provided basis and foundation to further study of P.americana.