Observation on the biological behavior of acute leukemia bone marrow stroma cells

Objective To study the mechanism of acute leukemic bone marrow stroma in leukemia development.Methods With the method of bone marrow stromal cell culture system or co-culture system of bone marrow stromal cells, investigate the growth properties of leukemic bone marrow stroma, the expression of VCAM-1(vascular cell adhesion molecule-1),Fn(fibronectin) and the content of IL-6(interleukin-6),TNF-α(tumor necrosis factor-alpha) in culture medium.Results The growth of acute leukemic bone marrow stromal cells was delayed. The time of sticking to wall, the time of microplexus formation, the time of colony formation, the time of confluence formation, were all significantly delayed (P0.05). The number of CFU-F and the content of VCAM-1 in stromal cells was respectively significantly decreased with that of controls(P0.05),in addition, the content of Fn in stromal cells from acute leukemia was identical with that of controls(P0.05),whereas, the content of IL-6 and TNF-α in the culture medium from acute leukemic bone marrow stromal cells was significantly increased compared with that of controls.Conclusion There were many abnormalities in hematopoietic inductive microenvironment(HIM)of acute leukemia.