For making known to the content and composition of fatty acids in plant oil and then offering reference to the preparation of nutritional blending oil,we make the analysis on fatty acids content and the variation of the constructions of fatty acids in several kinds of plant oil with Gas Chromatography(GC) after NaOCH3 methylation.The results show that plant oil contains large amount of unsaturated fatty acids,mostly are oleic acid and linoleic acid,a small amount of Conjugated Linoleic Acids(CLA) also are found in plant oil,saturated fatty acids mostly are palmitoleic acid and stearic acid.The ratio of n-6/n-3 fatty acids in mostly plant oil are great that range from 11.15 to 286.37 but in linseed oil and refining rape oil is comparatively smaller,0.44 and 2.84 respectively.